| First Author | Binder RJ | Year | 2005 |
| Journal | Nat Immunol | Volume | 6 |
| Issue | 6 | Pages | 593-9 |
| PubMed ID | 15864309 | Mgi Jnum | J:98952 |
| Mgi Id | MGI:3580767 | Doi | 10.1038/ni1201 |
| Citation | Binder RJ, et al. (2005) Peptides chaperoned by heat-shock proteins are a necessary and sufficient source of antigen in the cross-priming of CD8+ T cells. Nat Immunol 6(6):593-9 |
| abstractText | The form in which antigens are transferred from cancer cells or infected cells to antigen-presenting cells as a part of the process of priming CD8(+) T cells has been a longstanding unresolved issue. Intact proteins or protein fragments in the form of free peptides or peptides chaperoned by heat-shock protein are possible sources of antigen. We address this here using beta-galactosidase and ovalbumin. Immunization with cell lysates containing intact proteins and heat-shock protein-peptide complexes or with cell lysates depleted of either component demonstrated that protein fragments chaperoned by heat-shock protein and not intact protein were the necessary and sufficient source of antigen transferred to antigen-presenting cells for priming CD8(+) T cell responses. |
