| First Author | Nieland TJ | Year | 2005 |
| Journal | Biochim Biophys Acta | Volume | 1734 |
| Issue | 1 | Pages | 44-51 |
| PubMed ID | 15866482 | Mgi Jnum | J:99075 |
| Mgi Id | MGI:3581083 | Doi | 10.1016/j.bbalip.2005.02.007 |
| Citation | Nieland TJ, et al. (2005) Endocytosis is not required for the selective lipid uptake mediated by murine SR-BI. Biochim Biophys Acta 1734(1):44-51 |
| abstractText | The scavenger receptor class B, type I (SR-BI) mediates the cellular selective uptake of cholesteryl esters and other lipids from high-density lipoproteins (HDL) and low-density lipoproteins (LDL). This process, unlike classical receptor-mediated endocytosis, does not result in lipoprotein degradation. Instead, the lipid depleted particles are released into the medium. Here we show that selective lipid uptake mediated by murine SR-BI can be uncoupled from the endocytosis of HDL or LDL particles. We found that blocking selective lipid uptake by incubating cells with the small chemical inhibitors BLT-1 or BLT-4 did not affect endocytosis of HDL. Similarly, blocking endocytosis by hyperosmotic sucrose or K+ depletion did not prevent selective lipid uptake from HDL or LDL. These findings suggest that mSR-BI-mediated selective uptake occurs at the cell surface upon the association of lipoproteins with mSR-BI and does not require endocytosis of HDL or LDL particles. |
