| First Author | Torero Ibad R | Year | 2020 |
| Journal | eNeuro | Volume | 7 |
| Issue | 5 | PubMed ID | 32737182 |
| Mgi Jnum | J:302433 | Mgi Id | MGI:6508283 |
| Doi | 10.1523/ENEURO.0012-19.2020 | Citation | Torero Ibad R, et al. (2020) OTX2 Non-Cell Autonomous Activity Regulates Inner Retinal Function. eNeuro 7(5):ENEURO.0012-19.2020 |
| abstractText | OTX2 is a homeoprotein transcription factor expressed in photoreceptors and bipolar cells in the retina. OTX2, like many other homeoproteins, transfers between cells and exerts non-cell autonomous effects such as promoting the survival of retinal ganglion cells that do not express the protein. Here we used a genetic approach to target extracellular OTX2 in the retina by conditional expression of a secreted single-chain anti-OTX2 antibody. Compared with control mice, the expression of this antibody by parvalbumin-expressing neurons in the retina is followed by a reduction in visual acuity in 1-month-old mice with no alteration of the retinal structure or cell type number or aspect. The a-waves and b-waves measured by electroretinogram were also indistinguishable from those of control mice, suggesting no functional deficit of photoreceptors and bipolar cells. Mice expressing the OTX2-neutralizing antibody did show a significant doubling in the flicker amplitude and a reduction in oscillatory potential, consistent with a change in inner retinal function. Our results show that interfering in vivo with OTX2 non-cell autonomous activity in the postnatal retina leads to an alteration in inner retinal cell functions and causes a deficit in visual acuity. |
