| First Author | Hashimoto A | Year | 2005 |
| Journal | J Biol Chem | Volume | 280 |
| Issue | 28 | Pages | 26225-32 |
| PubMed ID | 15901741 | Mgi Jnum | J:100838 |
| Mgi Id | MGI:3589716 | Doi | 10.1074/jbc.M409363200 |
| Citation | Hashimoto A, et al. (2005) BAD detects coincidence of G2/M phase and growth factor deprivation to regulate apoptosis. J Biol Chem 280(28):26225-32 |
| abstractText | BAD, a member of the Bcl-2 protein family, promotes mitochondria-dependent apoptosis. Here, we report that BAD dissociates from 14-3-3zeta at each G2/M phase of proliferating lymphoid cells. The cell cycle-dependent dissociation of BAD was associated with phosphorylation at Ser-128, whereas mutant S128A-BAD, in which Ser-128 was converted to alanine, remained associated with 14-3-3zeta throughout the cell cycle. Although the cell cycle-dependent dissociation of BAD per se did not induce apoptosis, growth factor deprivation induced prompt apoptosis at the G2/M phase but not at the G1 phase. In cells expressing S128A-BAD, growth factor deprivation-induced apoptosis was markedly delayed and was accompanied by a delayed dephosphorylation of growth factor-dependent regulatory serine residues. These results indicate that BAD induces apoptosis upon detecting the coincidence of G2/M phase and growth factor deprivation. |
