| First Author | Hashimoto J | Year | 2005 |
| Journal | Exp Cell Res | Volume | 310 |
| Issue | 2 | Pages | 256-69 |
| PubMed ID | 16165127 | Mgi Jnum | J:102792 |
| Mgi Id | MGI:3608075 | Doi | 10.1016/j.yexcr.2005.07.025 |
| Citation | Hashimoto J, et al. (2005) Laminin-5 suppresses chondrogenic differentiation of murine teratocarcinoma cell line ATDC5. Exp Cell Res 310(2):256-69 |
| abstractText | Laminin-5 is an important basement membrane protein that regulates cell adhesion and motility. It was previously found that the gamma2 chain of laminin-5 is transiently expressed in embryonic cartilage. This suggests a possible role of laminin-5 in chondrogenesis. Here, we examined this possibility using the murine teratocarcinoma cell line ATDC5. ATDC5 cells transiently and weakly expressed laminin-5 when they were stimulated for differentiation. Exogenous laminin-5 in either insoluble or soluble form strongly inhibited the differentiation phenotypes, i.e. formation of cartilaginous cell aggregates and production of chondrogenic marker proteins through its integrin-binding domain LG3 in the alpha3 chain. Laminin-5 had no effect on cell growth. In addition, we found that the laminin-5 with the 105-kDa, processed gamma2 chain suppressed differentiation more strongly than one with the 150-kDa gamma2 chain. This indicated that the proteolytic processing of gamma2 chain regulated the activity of laminin-5. However, a gamma2 chain short arm fragment had no effect on the chondrogenesis, and it rather suppressed the differentiation at excessive concentrations. These results suggest that laminin-5 and its processing modulate chondrogenic differentiation during development. |
