| First Author | Dupuy AJ | Year | 2002 |
| Journal | Proc Natl Acad Sci U S A | Volume | 99 |
| Issue | 7 | Pages | 4495-9 |
| PubMed ID | 11904379 | Mgi Jnum | J:104040 |
| Mgi Id | MGI:3611075 | Doi | 10.1073/pnas.062630599 |
| Citation | Dupuy AJ, et al. (2002) Mammalian germ-line transgenesis by transposition. Proc Natl Acad Sci U S A 99(7):4495-9 |
| abstractText | Transposons have been used in invertebrates for transgenesis and insertional mutagens in genetic screens. We tested a functional transposon called Sleeping Beauty in the one-cell mouse embryo. In this report, we describe experiments in which transposon vectors were injected into one-cell mouse embryos with mRNA expressing the SB10 transposase enzyme. Molecular evidence of transposition was obtained by cloning of insertion sites from multiple transgenic mice produced by SB10 mRNA/transposon coinjection. We also demonstrate germ-line transmission and expression from transposed elements. This technique has promise as a germ-line transgenesis method in other vertebrate species and for insertional mutagenesis in the mouse. |
