| First Author | Omoteyama K | Year | 2006 |
| Journal | Biochem Biophys Res Commun | Volume | 339 |
| Issue | 4 | Pages | 1089-97 |
| PubMed ID | 16343439 | Mgi Jnum | J:104218 |
| Mgi Id | MGI:3611526 | Doi | 10.1016/j.bbrc.2005.11.119 |
| Citation | Omoteyama K, et al. (2006) Activation of connective tissue growth factor gene by the c-Maf and Lc-Maf transcription factors. Biochem Biophys Res Commun 339(4):1089-97 |
| abstractText | The Maf family of transcription factors is expressed during development of various organs and tissues, and is involved in a variety of developmental and cellular differentiation processes. We previously found that c-maf and mafB are strongly expressed in hypertrophic chondrocytes during cartilage development. Connective tissue growth factor (CTGF) is also expressed in hypertrophic chondrocytes. Adenovirus mediated introduction of c-maf gene into the mouse fibroblast cell line C3H10T1/2 strongly induced CTGF expression. CTGF can be induced by TGF-beta via the SMAD pathway; however, the c-Maf could not induce TGF-beta, nor could TGF-beta induce the c-Maf, suggesting that activation of CTGF by Maf is TGF-beta independent. Reporter transfection analysis using C3H10T1/2 cells shows that c-Maf stimulates a CTGF reporter gene. Lc-Maf, a splice variant of c-Maf containing an extra 10 amino acids in the carboxyl terminus, was a stronger inducer of the CTGF reporter gene than c-Maf. Chromatin immunoprecipitation analysis showed that c-Maf binds to the promoter region of the CTGF gene, indicating that Maf directly activates the CTGF gene. Taken together, these data indicate that the CTGF gene is a target of c-Maf and Lc-Maf in cartilage development. |
