| First Author | Ruf N | Year | 2006 |
| Journal | Genomics | Volume | 87 |
| Issue | 4 | Pages | 509-19 |
| PubMed ID | 16455231 | Mgi Jnum | J:108174 |
| Mgi Id | MGI:3623175 | Doi | 10.1016/j.ygeno.2005.12.007 |
| Citation | Ruf N, et al. (2006) Expression profiling of uniparental mouse embryos is inefficient in identifying novel imprinted genes. Genomics 87(4):509-19 |
| abstractText | Imprinted genes are expressed from only one allele in a parent-of-origin-specific manner. We here describe a systematic approach to identify novel imprinted genes using quantification of allele-specific expression by Pyrosequencing, a highly accurate method to detect allele-specific expression differences. Sixty-eight candidate imprinted transcripts mapping to known imprinted chromosomal regions were selected from a recent expression profiling study of uniparental mouse embryos and analyzed. Three novel imprinted transcripts encoding putative non-protein-coding RNAs were identified on the basis of parent-of-origin-specific monoallelic expression in E11.5 (C57BL/6 x Cast/Ei)F1 and informative (C57BL/6 x Cast/Ei) x C57BL/6 backcross embryos. In addition, four transcripts with preferential expression of a strain-specific allele were found. Intriguingly, a vast majority of the analyzed transcripts showed no imprinting-associated expression in F1 embryos. These data strengthen the view that a large fraction of nonimprinted genes is differentially expressed between parthenogenetic and androgenetic embryos and question the efficiency of expression profiling of uniparental embryos to identify novel imprinted genes. |
