| First Author | Issa LL | Year | 2006 |
| Journal | Dev Biol | Volume | 293 |
| Issue | 1 | Pages | 104-15 |
| PubMed ID | 16494860 | Mgi Jnum | J:108465 |
| Mgi Id | MGI:3624078 | Doi | 10.1016/j.ydbio.2006.01.019 |
| Citation | Issa LL, et al. (2006) MusTRD can regulate postnatal fiber-specific expression. Dev Biol 293(1):104-15 |
| abstractText | Human MusTRD1alpha1 was isolated as a result of its ability to bind a critical element within the Troponin I slow upstream enhancer (TnIslow USE) and was predicted to be a regulator of slow fiber-specific genes. To test this hypothesis in vivo, we generated transgenic mice expressing hMusTRD1alpha1 in skeletal muscle. Adult transgenic mice show a complete loss of slow fibers and a concomitant replacement by fast IIA fibers, resulting in postural muscle weakness. However, developmental analysis demonstrates that transgene expression has no impact on embryonic patterning of slow fibers but causes a gradual postnatal slow to fast fiber conversion. This conversion was underpinned by a demonstrable repression of many slow fiber-specific genes, whereas fast fiber-specific gene expression was either unchanged or enhanced. These data are consistent with our initial predictions for hMusTRD1alpha1 and suggest that slow fiber genes contain a specific common regulatory element that can be targeted by MusTRD proteins. |
