| First Author | Mullins JJ | Year | 1989 |
| Journal | EMBO J | Volume | 8 |
| Issue | 13 | Pages | 4065-72 |
| PubMed ID | 2480233 | Mgi Jnum | J:328034 |
| Mgi Id | MGI:6727490 | Doi | 10.1002/j.1460-2075.1989.tb08590.x |
| Citation | Mullins JJ, et al. (1989) Expression of the DBA/2J Ren-2 gene in the adrenal gland of transgenic mice. EMBO J 8(13):4065-72 |
| abstractText | To characterize further the tissue-specific control of the mouse Ren-2 gene, and in particular its expression in the adrenal gland, we have introduced the DBA/2 Ren-2 gene into the genome of Ren-1c/Ren-1c mice. Here we report our observations on Ren-2 transgenic mice. Expression was found in the correct spectrum of tissues and included appropriate hormonal control in the submandibular gland. Quantitatively transcript levels varied both positively (adrenal gland and sex-accessory tissue) and negatively (submandibular gland and kidney) with respect to normal Ren-2 expression. In the DBA/2 inbred mouse strain expression in the female adrenal gland cycles during oestrus between the X-zone and the zona fasciculata. Transgene expression within the adrenal gland was restricted to the X-zone. Therefore this phenotype, which is characteristic of most two-renin-gene strains of mice, contrasts with that found in the strain DBA/2 from which the transgene was derived. This suggests that cell-specific expression in the DBA/2 adrenal gland is mediated in trans by at least one additional locus. We demonstrate that suitable genetic crosses of the transgenic mice can partially restore the cycling phenotype. |
