| First Author | Kloosterman WP | Year | 2006 |
| Journal | Nat Methods | Volume | 3 |
| Issue | 1 | Pages | 27-9 |
| PubMed ID | 16369549 | Mgi Jnum | J:112756 |
| Mgi Id | MGI:3663527 | Doi | 10.1038/nmeth843 |
| Citation | Kloosterman WP, et al. (2006) In situ detection of miRNAs in animal embryos using LNA-modified oligonucleotide probes. Nat Methods 3(1):27-9 |
| abstractText | MicroRNAs (miRNAs) are 20-23 nucleotide (nt) RNA molecules that regulate gene expression post-transcriptionally. A key step toward understanding the function of the hundreds of miRNAs identified in animals is to determine their expression during development. Here we performed a detailed analysis of conditions for in situ detection of miRNAs in the zebrafish embryo using locked nucleic acid (LNA)-modified DNA probes and report expression patterns for 15 miRNAs in the mouse embryo. |
