| First Author | Rhee JW | Year | 2007 |
| Journal | Mol Immunol | Volume | 44 |
| Issue | 6 | Pages | 1393-400 |
| PubMed ID | 16780951 | Mgi Jnum | J:113217 |
| Mgi Id | MGI:3664816 | Doi | 10.1016/j.molimm.2006.05.003 |
| Citation | Rhee JW, et al. (2007) Regulation of matrix metalloproteinase-9 gene expression and cell migration by NF-kappaB in response to CpG-oligodeoxynucleotides in RAW 264.7 cells. Mol Immunol 44(6):1393-400 |
| abstractText | Matrix metalloproteinase-9 (MMP-9) is a secreted type IV collagenase that plays an important role in the remodeling of the extracellular matrix (ECM) and the migration of normal and tumor cells. We have shown that CpG-ODN-induced migration of RAW 264.7 cell is regulated by MMP-9 activity by using tissue inhibitors of MMP-1 (TIMP-1). The MMP-9 gene expression was transcriptionally induced by CpG-ODN in a time-dependent manner. An MMP-9 promoter-reporter was activated by the stimulation of CpG-ODN and ectopical expression of NF-kappaB transcription factor. Inhibition of NF-kappaB nuclear localization by co-expression of a mutant IkappaBalpha protein blocked the CpG-ODN-induced MMP-9 promoter activation. BMS-345541, an IKK-2 inhibitor also inhibited the expression of MMP-9 gene induced by CpG-ODN. Direct binding of NF-kappaB protein to the promoter region of the MMP-9 was confirmed by chromatin immunoprecipitation using NF-kappaB antibody. These results lead us to a conclusion that NF-kappaB activation is required for MMP-9 gene expression. In summary, our data suggest that NF-kappaB-dependent expression of MMP-9 in response to CpG-ODN plays an important role in the recruitment of immune cells. |
