| First Author | Nakadai T | Year | 1999 |
| Journal | DNA Res | Volume | 6 |
| Issue | 3 | Pages | 207-9 |
| PubMed ID | 10470852 | Mgi Jnum | J:113787 |
| Mgi Id | MGI:3687668 | Doi | 10.1093/dnares/6.3.207 |
| Citation | Nakadai T, et al. (1999) Structure of rat gamma-tubulin and its binding to HP33. DNA Res 6(3):207-9 |
| abstractText | Gamma-tubulin is localized at the microtubule organizing center and is thought to participate in the organizing of the microtubule network. In this study, we isolated a cDNA of rat gamma-tubulin. The rat gamma-tubulin cDNA encoded 451 amino acids, the same number as that of its counterpart in other vertebrates, and its structure was found to be highly conserved in vertebrates. In a previous work, we identified HP33 (hepatocarcinogenesis- and hepatocellular proliferation-related 33-kDa protein) that was localized at the centrosome of hepatic cells and that exhibited MAP-like activity. In vitro GST pull-down assay using highly purified recombinant HP33 and bacterially expressed gamma-tubulin demonstrated that HP33 bound to gamma-tubulin directly. These results suggest that HP33 is localized at the centrosome via association with both the microtubule and its minus end-specific component, gamma-tubulin. |
