| First Author | Chen-Roetling J | Year | 2006 |
| Journal | Biochem Biophys Res Commun | Volume | 350 |
| Issue | 1 | Pages | 233-7 |
| PubMed ID | 16999934 | Mgi Jnum | J:114530 |
| Mgi Id | MGI:3689273 | Doi | 10.1016/j.bbrc.2006.09.036 |
| Citation | Chen-Roetling J, et al. (2006) Effect of heme oxygenase-1 on the vulnerability of astrocytes and neurons to hemoglobin. Biochem Biophys Res Commun 350(1):233-7 |
| abstractText | The heme oxygenase (HO) enzymes catalyze the rate-limiting step of heme breakdown. Prior studies have demonstrated that the vulnerability of neurons and astrocytes to hemoglobin is modified in cells lacking HO-2, the constitutive isoform. The present study assessed the effect of the inducible isoform, HO-1. Wild-type astrocytes treated for 3-5 days with 3-30 microM hemoglobin sustained no loss of viability, as quantified by LDH and MTT assays. The same treatment resulted in death of 25-50% of HO-1 knockout astrocytes, and a 4-fold increase in protein oxidation. Cell injury was attenuated by transfer of the HO-1 gene, but not by bilirubin, the antioxidant heme breakdown product. Conversely, neuronal protein oxidation and cell death after hemoglobin exposure were similar in wild-type and HO-1 knockout cultures. These results suggest that HO-1 induction protects astrocytes from the oxidative toxicity of Hb, but has no effect on neuronal injury. |
