| First Author | Sun Q | Year | 2006 |
| Journal | World J Gastroenterol | Volume | 12 |
| Issue | 17 | Pages | 2785-8 |
| PubMed ID | 16718771 | Mgi Jnum | J:115073 |
| Mgi Id | MGI:3690612 | Doi | 10.3748/wjg.v12.i17.2785 |
| Citation | Sun Q, et al. (2006) Generation and characterization of a transgenic mouse model for pancreatic cancer. World J Gastroenterol 12(17):2785-8 |
| abstractText | AIM: To generate a SV40Tag transgenic tumor animal model and to study the mechanism underlying tumorigenesis. METHODS: A mammary gland expression vector containing SV40Tag DNA was generated. Transgene fragments were microinjeted into fertilized eggs of FVB mice. The genetically manipulated embryos were transferred into the oviducts of pseudo-pregnant female mice. PCR and Northern blot analysis were used for genotype analysis of F1 and F2 mice. Transgene expression was detected by RT-PCR and immunohistochemistry. RESULTS: SV40Tag gene was detected in two lines of transgenic mice. One of them delivered the transgene to F1 and a tumor was found in the pancreas of these mice. RT-PCR and immunohistochemistry showed that SV40Tag gene was expressed in the tumor. Pathological characterization of the transgenic mice demonstrated that the tumor belonged to pancreatic cystic neoplasm. CONCLUSION: SV40Tag transgenic mouse model can be successfully established. The transgenic mice develop a pancreatic tumor, which can be used for investigation of the molecular mechanism of tumorigenesis in vivo. |
