| First Author | Visintin A | Year | 2006 |
| Journal | J Leukoc Biol | Volume | 80 |
| Issue | 6 | Pages | 1584-92 |
| PubMed ID | 16946018 | Mgi Jnum | J:116588 |
| Mgi Id | MGI:3694538 | Doi | 10.1189/jlb.0606388 |
| Citation | Visintin A, et al. (2006) MD-2 expression is not required for cell surface targeting of Toll-like receptor 4 (TLR4). J Leukoc Biol 80(6):1584-92 |
| abstractText | The cell surface receptor complex formed by TLR4 and myeloid differentiation 2 (MD-2) is engaged when cells are exposed to LPS. Recent studies suggested that surface localization of functional mouse TLR4 (mTLR4) depends on the simultaneous expression of MD-2. As we did not observe a similar requirement, we conducted a comparative study of human TLR4 and mTLR4 surface expression in immune cells derived from the MD-2 knockout mouse and LPS-responsive cell lines and in cells that ectopically express TLR4. Our results indicate that in the human and mouse models, neither TLR4 function nor TLR4 surface targeting requires MD-2 coexpression. Accordingly, we report on one human cell line, which constitutively expresses functional TLR4 on the cell surface in the absence of MD-2 expression. |
