| First Author | Chung DH | Year | 1999 |
| Journal | J Immunol | Volume | 163 |
| Issue | 7 | Pages | 3699-708 |
| PubMed ID | 10490965 | Mgi Jnum | J:118970 |
| Mgi Id | MGI:3700881 | Doi | 10.4049/jimmunol.163.7.3699 |
| Citation | Chung DH, et al. (1999) NK and CTL recognition of a single chain H-2Dd molecule: distinct sites of H-2Dd interact with NK and TCR. J Immunol 163(7):3699-708 |
| abstractText | We generated transgenic mice expressing a single-chain beta2-microglobulin (beta2m)-H-2Dd. The cell-surface beta2m-H-2Dd molecule was expressed on a beta2m-deficient background and reacted with appropriate mAbs. It was of the expected m.w. and directed the normal development of CD8+ T cells in the thymus of a broad TCR repertoire. It also presented both exogenously provided and endogenous peptide Ags to effector CD8+ T cells. In tests of NK cell education and function, it failed to reveal any interaction with NK cells, suggesting that the site of the interaction of NK receptors with H-2Dd was disrupted. Thus, the sites of TCR and NK receptor interaction with H-2Dd are distinct, an observation consistent with independent modes of TCR and NK receptor evolution and function. |
