| First Author | Kawashima T | Year | 2006 |
| Journal | J Cell Biol | Volume | 175 |
| Issue | 6 | Pages | 937-46 |
| PubMed ID | 17178910 | Mgi Jnum | J:119092 |
| Mgi Id | MGI:3701164 | Doi | 10.1083/jcb.200604073 |
| Citation | Kawashima T, et al. (2006) Rac1 and a GTPase-activating protein, MgcRacGAP, are required for nuclear translocation of STAT transcription factors. J Cell Biol 175(6):937-46 |
| abstractText | STAT transcription factors are tyrosine phosphorylated upon cytokine stimulation and enter the nucleus to activate target genes. We show that Rac1 and a GTPase-activating protein, MgcRacGAP, bind directly to p-STAT5A and are required to promote its nuclear translocation. Using permeabilized cells, we find that nuclear translocation of purified p-STAT5A is dependent on the addition of GTP-bound Rac1, MgcRacGAP, importin alpha, and importin beta. p-STAT3 also enters the nucleus via this transport machinery, and mutant STATs lacking the MgcRacGAP binding site do not enter the nucleus even after phosphorylation. We conclude that GTP-bound Rac1 and MgcRacGAP function as a nuclear transport chaperone for activated STATs. |
