| First Author | Zhao L | Year | 2007 |
| Journal | Mol Reprod Dev | Volume | 74 |
| Issue | 6 | Pages | 750-7 |
| PubMed ID | 17133603 | Mgi Jnum | J:122623 |
| Mgi Id | MGI:3714890 | Doi | 10.1002/mrd.20660 |
| Citation | Zhao L, et al. (2007) Dynamin 2 associates with complexins and is found in the acrosomal region of mammalian sperm. Mol Reprod Dev 74(6):750-7 |
| abstractText | Previous data showed that complexin I, a SNARE regulatory protein, is localized in and/or around the acrosome and is necessary for the acrosome reaction in sperm. To understand how complexin I regulates the acrosome reaction, we used complexin-GST pulldown assays to identify interacting proteins. We showed that both complexins I and II bound mouse sperm dynamin 2. Dynamin 2 is a 100 kDa GTPase essential to many aspects of endocytosis but its potential role in exocytosis is unknown. Dynamin 2 is expressed in rat testis and widely expressed in other tissues; however, the function of dynamin 2 in germ cells is uncertain. Dynamin 2 protein was detected in mouse testis and was most abundant in or around the developing acrosome of spermatids. In addition, dynamin 2 was co-localized with complexin I in the acrosomal region of mammalian sperm. Its co-localization and interaction with complexin I suggest that dynamin 2 may play a role during acrosome formation and/or acrosomal exocytosis. |
