| First Author | Stevens S | Year | 2000 |
| Journal | J Immunol | Volume | 164 |
| Issue | 10 | Pages | 5306-12 |
| PubMed ID | 10799892 | Mgi Jnum | J:123435 |
| Mgi Id | MGI:3718299 | Doi | 10.4049/jimmunol.164.10.5306 |
| Citation | Stevens S, et al. (2000) Role of OCA-B in 3'-IgH enhancer function. J Immunol 164(10):5306-12 |
| abstractText | OCA-B (alternately called Bob1 and OBF-1) is a B cell-specific coactivator that interacts with the ubiquitously expressed Oct-1 and the B cell-restricted Oct-2 to activate transcription via the octamer site (5'-ATGCAAAT-3'). OCA-B-/- mice appear to undergo normal Ag-independent B cell maturation. However, Ag-dependent B cell differentiation, including germinal center formation, production of secondary Ig isotypes, and proliferation in response to surface Ig cross-linking, is greatly affected. We demonstrate that the observed reductions in expression of class-switched isotypes in OCA-B-/- mice may be due in part to deficiencies in the function of the 3'-IgH enhancer elements. Furthermore, we find that surface Ig cross-linking represses all the Ig enhancers and that this repression is absent in OCA-B-/- B cells. These results suggest an important role for OCA-B in Ig enhancer function in vivo. |
