| First Author | Son YM | Year | 2008 |
| Journal | Biochem Biophys Res Commun | Volume | 365 |
| Issue | 1 | Pages | 113-7 |
| PubMed ID | 17980153 | Mgi Jnum | J:128511 |
| Mgi Id | MGI:3767363 | Doi | 10.1016/j.bbrc.2007.10.132 |
| Citation | Son YM, et al. (2008) Modulation of RAG/DNA complex by HSP70 in V(D)J recombination. Biochem Biophys Res Commun 365(1):113-7 |
| abstractText | V(D)J recombination, a site-specific gene rearrangement process, requires two RAG1 and RAG2 proteins specifically recognizing recombination signal sequences and forming DNA double-strand breaks. The broken DNA ends tightly bound to RAG proteins are joined by repair proteins. Here, we found that heat shock protein 70 was associated with RAG2 following two-step affinity chromatography purification. It was also co-immunoprecipitated with RAG2 in pro-B cells. Purified HSP70 protein disrupted RAG/DNA complexes assembled in vitro and also inhibited the V(D)J cleavage (both nick and hairpin formation) in a dose-dependent manner. This HSP70 action required ATP energy. These data suggest that HSP70 might play a crucial role in disassembling RAG/DNA complexes stably formed during V(D)J recombination. |
