| First Author | Selden RF | Year | 1988 |
| Journal | Proc Natl Acad Sci U S A | Volume | 85 |
| Issue | 21 | Pages | 8241-5 |
| PubMed ID | 3186721 | Mgi Jnum | J:129441 |
| Mgi Id | MGI:3769278 | Doi | 10.1073/pnas.85.21.8241 |
| Citation | Selden RF, et al. (1988) Expression of the human growth hormone variant gene in cultured fibroblasts and transgenic mice. Proc Natl Acad Sci U S A 85(21):8241-5 |
| abstractText | The nucleotide sequence of the human growth hormone variant gene, one of the five members of the growth hormone gene family, predicts that it encodes a growth hormone-like protein. As a first step in determining whether this gene is functional in humans, we have expressed a mouse metallothionein I/human growth hormone variant fusion gene in mouse L cells and in transgenic mice. The growth hormone variant protein expressed in transiently transfected L cells is distinct from growth hormone itself with respect to reactivity with anti-growth hormone monoclonal antibodies, behavior during column chromatography, and isoelectric point. Transgenic mice expressing the growth hormone variant protein are 1.4- to 1.9-fold larger than nontransgenic controls, suggesting that the protein has growth-promoting properties. |
