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Publication : An optimized immunohistochemical method for detection of phosphorylated mitogen-activated protein kinases.

First Author  Teraishi T Year  2008
Journal  J Immunol Methods Volume  330
Issue  1-2 Pages  34-43
PubMed ID  18061203 Mgi Jnum  J:133397
Mgi Id  MGI:3778394 Doi  10.1016/j.jim.2007.10.014
Citation  Teraishi T, et al. (2008) An optimized immunohistochemical method for detection of phosphorylated mitogen-activated protein kinases. J Immunol Methods 330(1-2):34-43
abstractText  Study of the in vivo spatio-temporal localization of modified proteins is likely to become a major focus of proteomics research in the near future. In this study we optimized and tested an immunohistochemical procedure for detecting unstable phosphorylated mitogen-activated protein (MAP) kinases. Using our method, phosphorylated MAP kinases can be sensitively and reproducibly localized in the developing white matter of murine spinal cord on embryonic day 15. Our method is simple and effective, and so may be useful in future proteomics research.
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