| First Author | Itahana Y | Year | 2008 |
| Journal | Biochem Biophys Res Commun | Volume | 372 |
| Issue | 4 | Pages | 826-30 |
| PubMed ID | 18519032 | Mgi Jnum | J:137764 |
| Mgi Id | MGI:3802858 | Doi | 10.1016/j.bbrc.2008.05.139 |
| Citation | Itahana Y, et al. (2008) Expression of Id and ITF-2 genes in the mammary gland during pregnancy. Biochem Biophys Res Commun 372(4):826-30 |
| abstractText | Id-1 is one of the four related helix-loop-helix Id proteins that act as inhibitors of the basic helix-loop-helix (bHLH) transcription factors that control cell differentiation, development and carcinogenesis. We previously found that Id-1 regulated the growth, differentiation, apoptosis and invasion of mouse mammary epithelial cells in culture. Using the techniques of immunohistochemistry and in situ hybridization, we now show that Id-1 gene expression is specifically detected in the epithelial cells of growing ductal structures during early pregnancy. Using adjacent sections, we determined that Id-1 was expressed in keratin 8/18 positive cells. We also demonstrated that the up-regulation of Id-2 during late pregnancy correlated with the down-regulation of Id-1. Using the yeast-two hybrid system, we identified the bHLH transcription factors, ITF-2A and ITF-2B, as the Id-interacting proteins. The levels of expression of these two splice variants did not change during the transition from growing ductal structures to differentiated alveoli. Therefore Id-1 and Id-2, but not the ubiquitous bHLH proteins, appear to represent the key factors whose expression is modulated during different stages of pregnancy in mouse mammary glands. |
