| First Author | Tskvitaria-Fuller I | Year | 2006 |
| Journal | J Immunol | Volume | 177 |
| Issue | 3 | Pages | 1708-20 |
| PubMed ID | 16849480 | Mgi Jnum | J:137999 |
| Mgi Id | MGI:3803535 | Doi | 10.4049/jimmunol.177.3.1708 |
| Citation | Tskvitaria-Fuller I, et al. (2006) Specific patterns of Cdc42 activity are related to distinct elements of T cell polarization. J Immunol 177(3):1708-20 |
| abstractText | T cell polarization toward and within the cellular interface with an APC is critical for effective T cell activation. The Rho family GTPase Cdc42 is a central regulator of cellular polarization. Using live-cell imaging, we characterized the spatiotemporal patterns of Cdc42 activity and their physiological regulation. Using three independent means of experimental manipulation of Cdc42 activity, we established that Cdc42 is a critical regulator of T cell actin dynamics, TCR clustering, and cell cycle entry. Using quantification of three-dimensional data, we could relate distinct spatiotemporal patterns of Cdc42 activity to specific elements of T cell activation. This result suggests that Cdc42 activity in specific locations at specific times is most critical for its function in T cell activation. |
