| First Author | Du H | Year | 2008 |
| Journal | Mol Cell | Volume | 31 |
| Issue | 5 | Pages | 641-9 |
| PubMed ID | 18775324 | Mgi Jnum | J:138773 |
| Mgi Id | MGI:3806382 | Doi | 10.1016/j.molcel.2008.08.012 |
| Citation | Du H, et al. (2008) Activation of 12/23-RSS-dependent RAG cleavage by hSWI/SNF complex in the absence of transcription. Mol Cell 31(5):641-9 |
| abstractText | Maintenance of genomic integrity during antigen receptor gene rearrangements requires (1) regulated access of the V(D)J recombinase to specific loci and (2) generation of double-strand DNA breaks only after recognition of a pair of matched recombination signal sequences (RSSs). Here we recapitulate both key aspects of regulated recombinase accessibility in a cell-free system using plasmid substrates assembled into chromatin. We show that recruitment of the SWI/SNF chromatin-remodeling complex to both RSSs increases coupled cleavage by RAG1 and RAG2 proteins. SWI/SNF functions by altering local chromatin structure in the absence of RNA polymerase II-dependent transcription or histone modifications. These observations demonstrate a direct role for cis-sequence-regulated local chromatin remodeling in RAG1/2-dependent initiation of V(D)J recombination. |
