| First Author | Navarro P | Year | 2008 |
| Journal | Science | Volume | 321 |
| Issue | 5896 | Pages | 1693-5 |
| PubMed ID | 18802003 | Mgi Jnum | J:139644 |
| Mgi Id | MGI:3809324 | Doi | 10.1126/science.1160952 |
| Citation | Navarro P, et al. (2008) Molecular coupling of Xist regulation and pluripotency. Science 321(5896):1693-5 |
| abstractText | During mouse embryogenesis, reversion of imprinted X chromosome inactivation in the pluripotent inner cell mass of the female blastocyst is initiated by the repression of Xist from the paternal X chromosome. Here we report that key factors supporting pluripotency-Nanog, Oct3/4, and Sox2-bind within Xist intron 1 in undifferentiated embryonic stem (ES) cells. Whereas Nanog null ES cells display a reversible and moderate up-regulation of Xist in the absence of any apparent modification of Oct3/4 and Sox2 binding, the drastic release of all three factors from Xist intron 1 triggers rapid ectopic accumulation of Xist RNA. We conclude that the three main genetic factors underlying pluripotency cooperate to repress Xist and thus couple X inactivation reprogramming to the control of pluripotency during embryogenesis. |
