| First Author | Brunskill EW | Year | 2008 |
| Journal | Dev Cell | Volume | 15 |
| Issue | 5 | Pages | 781-91 |
| PubMed ID | 19000842 | Mgi Jnum | J:142800 |
| Mgi Id | MGI:3822211 | Doi | 10.1016/j.devcel.2008.09.007 |
| Citation | Brunskill EW, et al. (2008) Atlas of gene expression in the developing kidney at microanatomic resolution. (Erratum: Dev Cell 2009; 16:482). Dev Cell 15(5):781-91 |
| abstractText | Kidney development is based on differential cell-type-specific expression of a vast number of genes. While multiple critical genes and pathways have been elucidated, a genome-wide analysis of gene expression within individual cellular and anatomic structures is lacking. Accomplishing this could provide significant new insights into fundamental developmental mechanisms such as mesenchymal-epithelial transition, inductive signaling, branching morphogenesis, and segmentation. We describe here a comprehensive gene expression atlas of the developing mouse kidney based on the isolation of each major compartment by either laser capture microdissection or fluorescence-activated cell sorting, followed by microarray profiling. The resulting data agree with known expression patterns and additional in situ hybridizations. This kidney atlas allows a comprehensive analysis of the progression of gene expression states during nephrogenesis, as well as discovery of potential growth factor-receptor interactions. In addition, the results provide deeper insight into the genetic regulatory mechanisms of kidney development. |
