| First Author | Otsuka A | Year | 2009 |
| Journal | Biochem Biophys Res Commun | Volume | 378 |
| Issue | 3 | Pages | 478-82 |
| PubMed ID | 19032944 | Mgi Jnum | J:143063 |
| Mgi Id | MGI:3822709 | Doi | 10.1016/j.bbrc.2008.11.066 |
| Citation | Otsuka A, et al. (2009) Dynamin 2 is required for actin assembly in phagocytosis in Sertoli cells. Biochem Biophys Res Commun 378(3):478-82 |
| abstractText | Dynamin 2 has been reported to be implicated in phagocytosis. However, the mode of action of dynamin is poorly understood. In this study, we examined whether dynamin 2 participates in actin assembly during phagocytosis in Sertoli cells. In the presence of dynasore, a dynamin inhibitor, phagocytosis was reduced by 60-70% in Sertoli cells and macrophages. Scanning electron microscopy revealed that Sertoli cells treated with dynasore were unable to form phagocytic cups. In addition, dysfunction of dynamin 2 reduced both actin polymerization and recruitment of actin and dynamin 2 to phosphatidylinositol (4,5) bisphosphate [PI(4,5)P(2)]-containing liposomes. The formation of dynamin 2-positive ruffles of Sertoli cells was decreased by 60-70% by sequestering PI(4,5)P(2) either by expression of PH domain of PLCdelta or treatment with neomycin. These results strongly suggest that dynamin 2 is involved in actin dynamics and the formation of dynamin 2-positive ruffles during phagocytosis. |
