| First Author | Baillat D | Year | 2009 |
| Journal | Biochem Biophys Res Commun | Volume | 378 |
| Issue | 2 | Pages | 213-7 |
| PubMed ID | 19022222 | Mgi Jnum | J:143069 |
| Mgi Id | MGI:3822715 | Doi | 10.1016/j.bbrc.2008.11.035 |
| Citation | Baillat D, et al. (2009) Ets-1 binds cooperatively to the palindromic Ets-binding sites in the p53 promoter. Biochem Biophys Res Commun 378(2):213-7 |
| abstractText | Due to its autoinhibition for DNA binding, the Ets-1 transcription factor must interact with partners to enhance its affinity for DNA. In a study on the stromelysin-1 promoter, we showed that Ets-1 binds cooperatively to two Ets-binding sites (EBS) organized in palindrome, thereby circumventing the need for a binding partner to counteract autoinhibition. This leads to the formation of an Ets-1-DNA-Ets-1 ternary complex necessary for promoter activation. Here we show that Ets-1 also binds cooperatively to the EBS palindrome of the human p53 promoter, despite the presence of a degenerate EBS to which Ets-1 cannot otherwise bind. Transcriptional transactivation through this palindrome fully correlates to Ets-1 binding. Thus, the cooperative binding model that we initially proposed for the stromelysin-1 promoter may be a general mechanism of Ets-1 binding to palindromic EBS separated by 4bp and a way to counteract binding site degeneracy. |
