| First Author | Zhang J | Year | 2008 |
| Journal | Biochem Biophys Res Commun | Volume | 377 |
| Issue | 1 | Pages | 136-40 |
| PubMed ID | 18834857 | Mgi Jnum | J:143312 |
| Mgi Id | MGI:3826348 | Doi | 10.1016/j.bbrc.2008.09.089 |
| Citation | Zhang J, et al. (2008) The cell growth suppressor, mir-126, targets IRS-1. Biochem Biophys Res Commun 377(1):136-40 |
| abstractText | miRNAs are a family of approximately 22-nuleotide-long noncoding RNAs involved in the formation and progress of tumors. Since traditional methods for the detection of miRNAs expression have many disadvantages, we developed a simple method called polyA RT PCR. With this method, we detected a series of miRNAs and found that mir-126 is one of the miRNAs underexpressed in breast cancer cells. Flow cytometry analysis showed that mir-126 inhibited cell cycle progression from G1/G0 to S. Further studies revealed that mir-126 targeted IRS-1 at the translation level. Knocking down of IRS-1 suppresses cell growth in HEK293 and breast cancer cell MCF-7, which recapitulates the effects of mir-126. In conclusion, we developed a simple method for high-throughput screening of miRNAs and found that mir-126, a cell growth suppressor, targets IRS-1. |
