| First Author | Capanni C | Year | 2008 |
| Journal | Exp Cell Res | Volume | 314 |
| Issue | 20 | Pages | 3628-37 |
| PubMed ID | 18951892 | Mgi Jnum | J:144877 |
| Mgi Id | MGI:3832128 | Doi | 10.1016/j.yexcr.2008.09.026 |
| Citation | Capanni C, et al. (2008) Prelamin A is involved in early steps of muscle differentiation. Exp Cell Res 314(20):3628-37 |
| abstractText | Lamin A is a nuclear lamina constituent implicated in a number of human disorders including Emery-Dreifuss muscular dystrophy. Since increasing evidence suggests a role of the lamin A precursor in nuclear functions, we investigated the processing of prelamin A during differentiation of C2C12 mouse myoblasts. We show that both protein levels and cellular localization of prelamin A are modulated during myoblast activation. Similar changes of lamin A-binding proteins emerin and LAP2alpha were observed. Furthermore, prelamin A was found in a complex with LAP2alpha in differentiating myoblasts. Prelamin A accumulation in cycling myoblasts by expressing unprocessable mutants affected LAP2alpha and PCNA amount and increased caveolin 3 mRNA and protein levels, while accumulation of prelamin A in differentiated muscle cells following treatment with a farnesyl transferase inhibitor appeared to inhibit caveolin 3 expression. Our data provide evidence for a critical role of the lamin A precursor in the early steps of muscle cell differentiation. |
