| First Author | Hariri LP | Year | 2007 |
| Journal | Cancer Biol Ther | Volume | 6 |
| Issue | 11 | Pages | 1753-62 |
| PubMed ID | 17986850 | Mgi Jnum | J:133610 |
| Mgi Id | MGI:3778921 | Doi | 10.4161/cbt.6.11.4852 |
| Citation | Hariri LP, et al. (2007) Serial endoscopy in azoxymethane treated mice using ultra-high resolution optical coherence tomography. Cancer Biol Ther 6(11):1753-62 |
| abstractText | PURPOSE: Optical coherence tomography (OCT) is a minimally invasive, depth-resolved imaging tool that can be implemented in a small diameter endoscope for imaging mouse models of colorectal cancer (CRC). In this study, we utilized ultrahigh resolution (UHR) OCT to serially image the lower colon of azoxymethane (AOM) treated A/J mouse models of CRC in order to monitor the progression of neoplastic transformations and determine if OCT is capable of identifying early disease. EXPERIMENTAL DESIGN: Thirteen AOM treated A/J and two control A/J mice were surveyed at four timepoints (8, 14, 22 and 26 weeks post AOM treatment) using a 2.0 mm diameter UHR OCT endoscopic system with 3.2 microm axial and 4.4 microm lateral resolution. Histological samples obtained at the final timepoint served as the diagnostic reference. A blinded expert panel of mouse colon pathologists provided diagnoses from the OCT images based on criteria developed from a separate training set of OCT images. Panel results were compared to histological diagnoses assigned by a blinded pathologist. RESULTS: At the final imaging timepoint, 95% of adenomas and 23% of gastrointestinal neoplasias (38% protruding GINs and 9% non-protruding GINs) were correctly diagnosed. The panel identified 68% of disease foci (95% adenoma, 76% protruding GINs and 13% non-protruding GINs). Over the OCT imaging timepoints, disease progression followed a typical succession, with normal or GIN preceding adenoma. CONCLUSIONS: Endoscopic UHR OCT enabled accurate diagnosis of adenomas, identification of protruding GIN and non-destructive visualization of CRC progression, providing a tool for cancer research in animal models. |
