| First Author | Zou Y | Year | 2009 |
| Journal | Genesis | Volume | 47 |
| Issue | 3 | Pages | 142-54 |
| PubMed ID | 19165829 | Mgi Jnum | J:146895 |
| Mgi Id | MGI:3838894 | Doi | 10.1002/dvg.20470 |
| Citation | Zou Y, et al. (2009) A new mouse model for temporal- and tissue-specific control of extracellular superoxide dismutase. Genesis 47(3):142-54 |
| abstractText | The extracellular isoform of superoxide dismutase (EC-SOD, Sod3) plays a protective role against various diseases and injuries mediated by oxidative stress. To investigate the pathophysiological roles of EC-SOD, we generated tetracycline-inducible Sod3 transgenic mice and directed the tissue-specific expression of transgenes by crossing Sod3 transgenic mice with tissue-specific transactivator transgenics. Double transgenic mice with liver-specific expression of Sod3 showed increased EC-SOD levels predominantly in the plasma as the circulating form, whereas double transgenic mice with neuronal-specific expression expressed higher levels of EC-SOD in hippocampus and cortex with intact EC-SOD as the dominant form. EC-SOD protein levels also correlated well with increased SOD activities in double transgenic mice. In addition to enabling tissue-specific expression, the transgene expression can be quickly turned on and off by doxycycline supplementation in the mouse chow. This mouse model, thus, provides the flexibility for on-off control of transgene expression in multiple target tissues. |
