| First Author | Yoon S | Year | 2009 |
| Journal | Biochem Biophys Res Commun | Volume | 382 |
| Issue | 2 | Pages | 400-4 |
| PubMed ID | 19285955 | Mgi Jnum | J:147873 |
| Mgi Id | MGI:3842856 | Doi | 10.1016/j.bbrc.2009.03.037 |
| Citation | Yoon S, et al. (2009) IEX-1-induced cell death requires BIM and is modulated by MCL-1. Biochem Biophys Res Commun 382(2):400-4 |
| abstractText | MCL-1 (myeloid cell leukemia-1) is a distinguished and pivotal member of the pro-survival BCL-2 family of proteins, and we isolated IEX-1 (immediate early response gene X-1) as a MCL-1-interacting protein using the yeast two-hybrid system and confirmed their endogenous association in human cells. The underlying mechanisms by which IEX-1 affects cell survival and death are largely unknown. Ectopic expression of IEX-1-induced caspase-dependent apoptosis in 293T cells, and the response was significantly modulated by changes in the MCL-1 expression level in cells. Forced expression of IEX-1 was unable to induce cell death or to perturb mitochondrial membrane potential in BIM-depleted cells. Additionally, knockouts of NOXA or PUMA did not affect the activities of IEX-1, indicating that the pro-death action of IEX-1 specifically requires BIM. Our findings provide insight into a new regulatory circuit that controls cell death and survival by the coordinated action of MCL-1, IEX-1, and BIM. |
