| First Author | Andersen DC | Year | 2009 |
| Journal | FEBS Lett | Volume | 583 |
| Issue | 17 | Pages | 2947-53 |
| PubMed ID | 19665021 | Mgi Jnum | J:152900 |
| Mgi Id | MGI:4360405 | Doi | 10.1016/j.febslet.2009.08.002 |
| Citation | Andersen DC, et al. (2009) 'The preadipocyte factor' DLK1 marks adult mouse adipose tissue residing vascular cells that lack in vitro adipogenic differentiation potential. FEBS Lett 583(17):2947-53 |
| abstractText | Delta-like 1 (Dlk1) is expressed in 3T3-L1 preadipocytes and has frequently been referred to as 'the' preadipocyte marker, yet the phenotype of DLK1(+) cells in adipose tissue remains undetermined. Herein, we demonstrate that DLK1(+) cells encompass around 1-2% of the adult mouse adipose stromal vascular fraction (SVF). Unexpectedly, the DLK1(+)SVF population was enriched for cells expressing genes generally ascribed to the vascular lineage and did not possess any adipogenic differentiation potential in vitro. Instead, DLK1(+) cells comprised an immediate ability for cobblestone formation, generation of tube-like structures on matrigel, and uptake of Acetylated Low Density-Lipoprotein, all characteristics of endothelial cells. We therefore suggest that DLK1(+)SVF cells are of a vascular origin and not them-selves committed preadipocytes as assumed hitherto. |
