| First Author | Choi JW | Year | 2009 |
| Journal | J Cell Sci | Volume | 122 |
| Issue | Pt 15 | Pages | 2710-5 |
| PubMed ID | 19584093 | Mgi Jnum | J:153151 |
| Mgi Id | MGI:4361073 | Doi | 10.1242/jcs.049767 |
| Citation | Choi JW, et al. (2009) AIMP2 promotes TNFalpha-dependent apoptosis via ubiquitin-mediated degradation of TRAF2. J Cell Sci 122(Pt 15):2710-5 |
| abstractText | AIMP2 (aminoacyl-tRNA synthetase interacting multifunctional protein 2; also known as JTV-1) was first identified as p38 in a macromolecular protein complex that consisted of nine different aminoacyl-tRNA synthetases and two other auxiliary factors. AIMP2 also plays pivotal roles in the regulation of cell proliferation and death. Although AIMP2 was previously shown to augment TNFalpha-induced cell death, its working mechanism in this signal pathway was not understood. Here, we investigate the functional significance and mode of action of AIMP2 in TNFalpha signaling. TNFalpha-induced cell death was compromised in AIMP2-deficient or -suppressed cells and exogenous supplementation of AIMP2 augmented apoptotic sensitivity to TNFalpha signaling. This activity was confirmed by the AIMP2-dependent increase of IkappaB and suppression of NFkappaB. We found binding of AIMP2 to TRAF2, a key player in the TNFalpha signaling pathway. AIMP2 augmented the association of an E3 ubiquitin ligase, c-IAP1, with TRAF2, causing ubiquitin-dependent degradation of TRAF2. These findings suggest that AIMP2 can mediate the pro-apoptotic activity of TNFalpha via the downregulation of TRAF2 expression. |
