| First Author | Sing A | Year | 2009 |
| Journal | Cell | Volume | 138 |
| Issue | 5 | Pages | 885-97 |
| PubMed ID | 19737517 | Mgi Jnum | J:153894 |
| Mgi Id | MGI:4366586 | Doi | 10.1016/j.cell.2009.08.020 |
| Citation | Sing A, et al. (2009) A vertebrate Polycomb response element governs segmentation of the posterior hindbrain. Cell 138(5):885-97 |
| abstractText | Chromatin remodeling by Polycomb group (PcG) and trithorax group (trxG) proteins regulates gene expression in all metazoans. Two major complexes, Polycomb repressive complexes 1 and 2 (PRC1 and PRC2), are thought to mediate PcG-dependent repression in flies and mammals. In Drosophila, PcG/trxG protein complexes are recruited by PcG/trxG response elements (PREs). However, it has been unclear how PcG/trxG are recruited in vertebrates. Here we have identified a vertebrate PRE, PRE-kr, that regulates expression of the mouse MafB/Kreisler gene. PRE-kr recruits PcG proteins in flies and mouse F9 cells and represses gene expression in a PcG/trxG-dependent manner. PRC1 and 2 bind to a minimal PRE-kr region, which can recruit stable PRC1 binding but only weak PRC2 binding when introduced ectopically, suggesting that PRC1 and 2 have different binding requirements. Thus, we provide evidence that similar to invertebrates, PREs act as entry sites for PcG/trxG chromatin remodeling in vertebrates. |
