| First Author | Homer H | Year | 2009 |
| Journal | Science | Volume | 326 |
| Issue | 5955 | Pages | 991-4 |
| PubMed ID | 19965510 | Mgi Jnum | J:155048 |
| Mgi Id | MGI:4412201 | Doi | 10.1126/science.1175326 |
| Citation | Homer H, et al. (2009) A spindle assembly checkpoint protein functions in prophase I arrest and prometaphase progression. Science 326(5955):991-4 |
| abstractText | Two critical stages of mammalian oocyte regulation are prophase I arrest, which is important for sustaining the oocyte pool, and the progression through meiosis I (MI) to produce fertilizable eggs. We have found that the spindle assembly checkpoint protein BubR1 regulates both stages in mouse oocytes. We show that oocytes depleted of BubR1 cannot sustain prophase I arrest and readily undergo germinal vesicle breakdown, a marker for reentry into MI. BubR1-depleted oocytes then arrest before completing MI, marked by failure of polar body extrusion. Both meiotic defects in BubR1-depleted oocytes are due to reduced activity of the master regulator known as the anaphase-promoting complex (APC), brought about through diminished levels of the APC coactivator Cdh1. |
