| First Author | Yoon JH | Year | 2010 |
| Journal | Genes Dev | Volume | 24 |
| Issue | 2 | Pages | 123-8 |
| PubMed ID | 20080950 | Mgi Jnum | J:155933 |
| Mgi Id | MGI:4418372 | Doi | 10.1101/gad.1872810 |
| Citation | Yoon JH, et al. (2010) Error-free replicative bypass of (6-4) photoproducts by DNA polymerase zeta in mouse and human cells. Genes Dev 24(2):123-8 |
| abstractText | The ultraviolet (UV)-induced (6-4) pyrimidine-pyrimidone photoproduct [(6-4) PP] confers a large structural distortion in DNA. Here we examine in human cells the roles of translesion synthesis (TLS) DNA polymerases (Pols) in promoting replication through a (6-4) TT photoproduct carried on a duplex plasmid where bidirectional replication initiates from an origin of replication. We show that TLS contributes to a large fraction of lesion bypass and that it is mostly error-free. We find that, whereas Pol eta and Pol iota provide alternate pathways for mutagenic TLS, surprisingly, Pol zeta functions independently of these Pols and in a predominantly error-free manner. We verify and extend these observations in mouse cells and conclude that, in human cells, TLS during replication can be markedly error-free even opposite a highly distorting DNA lesion. |
