| First Author | Yoo W | Year | 2010 |
| Journal | Biochem Biophys Res Commun | Volume | 397 |
| Issue | 2 | Pages | 170-5 |
| PubMed ID | 20529675 | Mgi Jnum | J:162417 |
| Mgi Id | MGI:4818849 | Doi | 10.1016/j.bbrc.2010.05.067 |
| Citation | Yoo W, et al. (2010) Albumin expression is required for adipocyte differentiation of 3T3-L1 cells. Biochem Biophys Res Commun 397(2):170-5 |
| abstractText | We have previously demonstrated that albumin is directly involved in the formation of cytoplasmic lipid droplets in pancreatic stellate cells and may act as a downstream effector of adipogenic transcription factors, PPAR-gamma and C/EBP-alpha. Here, we investigated the role of albumin in adipocyte differentiation using 3T3-L1 cells. Albumin expression was significantly increased at later stages of adipocyte differentiation, which was accompanied with increased C/EBP-beta binding to albumin promoter. Suppression of albumin expression using short-hairpin RNA (shRNA) during differentiation led to a considerable reduction in lipid droplet formation, whereas albumin overexpression was stimulatory. Furthermore, point mutation in its fatty acid-binding sites inhibited lipid droplet formation. Consistent with these in vitro finding, Nagase analbuminemic rats displayed reduced fat accumulation. Therefore, our findings suggest that albumin may play a distinct role in adipocyte differentiation by promoting lipid accumulation. |
