| First Author | Tian H | Year | 2010 |
| Journal | Biochem Biophys Res Commun | Volume | 398 |
| Issue | 3 | Pages | 519-24 |
| PubMed ID | 20599763 | Mgi Jnum | J:163939 |
| Mgi Id | MGI:4830211 | Doi | 10.1016/j.bbrc.2010.06.111 |
| Citation | Tian H, et al. (2010) Beta-catenin/LEF1 activated enamelin expression in ameloblast-like cells. Biochem Biophys Res Commun 398(3):519-24 |
| abstractText | Enamelin is an ameloblast-specific matrix protein believed to play essential roles in enamel formation. However, mechanisms of enamelin transcription regulation are not clear. beta-Catenin/LEF1 is a key transcriptional complex involved in tooth development. In this study, the role of beta-catenin/LEF1 in enamelin expression was investigated. The 5'-flanking region of the mouse enamelin gene was analyzed and cloned. Co-transfection analysis and mutation assays revealed that two conserved LEF1 responsive elements located at -1002 and -597bp upstream of the enamelin translation initiation site could augment transcriptional activity of the enamelin. The interaction between the enamelin elements and beta-catenin/LEF1 was further confirmed by electrophoresis mobility shift assays and chromatin immunoprecipitation assays. In addition, LiCl treatment induced nuclear translocation of beta-catenin and elevated endogenous enamelin expression in mouse ameloblast-like cells. The results suggested that Wnt/beta-catenin signaling could function in enamelin gene expression by direct interaction through two conserved LEF1 responsive elements on the enamelin gene in ameloblast-like cells. |
