| First Author | Zheng W | Year | 2010 |
| Journal | Mol Cell Endocrinol | Volume | 330 |
| Issue | 1-2 | Pages | 59-71 |
| PubMed ID | 20797425 | Mgi Jnum | J:166631 |
| Mgi Id | MGI:4848268 | Doi | 10.1016/j.mce.2010.08.010 |
| Citation | Zheng W, et al. (2010) The orphan nuclear receptors COUP-TFI and COUP-TFII regulate expression of the gonadotropin LHbeta gene. Mol Cell Endocrinol 330(1-2):59-71 |
| abstractText | Normal sexual development and reproductive function depend on precise temporal and quantitative expression of the pituitary gonadotropins, LH and FSH. LHbeta-subunit gene expression is achieved by transcription factors acting at highly conserved and closely spaced cis-elements in the proximal 200 base pairs of the promoter. We now demonstrate that LHbeta promoter activity is further regulated by the orphan nuclear receptors, chicken ovalbumin upstream promoter-transcription factors (COUP-TFI and COUP-TFII). These data establish that COUP-TFs are expressed in primary pituitary gonadotropes and two gonadotrope-derived cell lines. COUP-TFs bind to two promoter regions in the LHbeta gene which overlap but are distinct from two previously defined cis-elements for another orphan nuclear receptor, steroidogenic factor-1 (SF-1). Transient transfection studies demonstrated that COUP-TFs stimulate LHbeta gene promoter activity in the absence of SF-1, but blunt SF-1-mediated stimulation of gene expression in a reporter construct containing both SF-1 cis-elements (GSEs). Evaluation of constructs containing mutations or truncations in the GSEs revealed a complex pattern of activation and inhibition by COUP-TF on this promoter, suggesting multiple mechanisms by which this factor modulates LHbeta gene expression. To our knowledge, these data are the first to demonstrate COUP-TF expression and function in pituitary gonadotropes. |
