| First Author | Lin Y | Year | 2011 |
| Journal | Am J Pathol | Volume | 178 |
| Issue | 2 | Pages | 506-14 |
| PubMed ID | 21281784 | Mgi Jnum | J:169086 |
| Mgi Id | MGI:4939839 | Doi | 10.1016/j.ajpath.2010.10.005 |
| Citation | Lin Y, et al. (2011) Reduced stathmin-1 expression in natural killer cells associated with spontaneous abortion. Am J Pathol 178(2):506-14 |
| abstractText | Female CBA/J mice impregnated by male DBA/2J mice (CBA/JxDBA/2J matings) are prone to spontaneous abortion, although the reason for this is unclear. In this study, the stathmin-1 expression pattern was evaluated in uterine natural killer (uNK) cells purified from CBA/JxDBA/2J matings. Results were compared with those in a CBA/JxBALB/c control group that yields successful pregnancies. The mean +/- SD percentage of stathmin-1(+) cells in the CD49b(+) uNK cell population was lower in CBA/JxDBA/2J mice (0.7% +/- 0.4%) than in control CBA/JxBALB/c mice (4.9% +/- 1.5%, P < 0.01) using flow cytometry, and the intracellular stathmin-1 level in uNK cells was lower in CBA/JxDBA/2J mice than in control mice using Western blot analysis. Co-localization of lectin from Dolichos biflorus agglutinin (DBA-lectin) and stathmin-1 was confirmed using multivision immunohistochemical analysis. The frequency of stathmin-1(+)DBA-lectin(+) cells was lower in CBA/JxDBA/2J mice than in CBA/JxBALB/c mice. A similar trend in the frequency of stathmin-1(+)CD56(+) cells was seen in patients with unexplained spontaneous abortion compared with normal early pregnancy. A neutralizing antibody against stathmin-1 further increased the percentage of embryo loss in CBA/JxDBA/2J matings. These results provide evidence that stathmin-1 expression in uNK cells at the maternal-fetal interface may help modulate uNK cell function and may be beneficial for a successful pregnancy. |
