| First Author | Russell JK | Year | 1990 |
| Journal | Biochem Biophys Res Commun | Volume | 168 |
| Issue | 2 | Pages | 696-701 |
| PubMed ID | 2159298 | Mgi Jnum | J:169822 |
| Mgi Id | MGI:4942275 | Doi | 10.1016/0006-291x(90)92377-c |
| Citation | Russell JK, et al. (1990) The I-A beta b region (65-85) is a binding site for the superantigen, staphylococcal enterotoxin A. Biochem Biophys Res Commun 168(2):696-701 |
| abstractText | Ia antigen is a receptor for the superantigen staphylococcal enterotoxin A (SEA). Peptides I-A beta b(30-60), I-A beta b(50-70), I-A beta b(65-85), and I-A beta b(80-100) of the MHC class II antigen beta chain on mouse (H-2b) accessory cells were synthesized. Only I-A beta b(65-85) inhibited SEA binding to the mouse B-cell lymphoma line, A20 (H-2d) and the human Burkitt's lymphoma line, Raji (HLA-DR). The I-A beta b(65-85) sequence is a predicted alpha-helix along the hypothetical antigen binding cleft of the Ia molecule. I-A beta b(65-85) also directly and specifically bound both the intact SEA molecule and its Ia binding site, represented by the peptide SEA(1-45). The results suggest that I-A beta b region (65-85) is a necessary site for Ia molecular interaction with the superantigen SEA. Further, the data suggest that the same helical region of other Ia antigens binds SEA irrespective of haplotype and species. |
