| First Author | Jo JO | Year | 2010 |
| Journal | Biochim Biophys Acta | Volume | 1803 |
| Issue | 11 | Pages | 1244-51 |
| PubMed ID | 20691219 | Mgi Jnum | J:170008 |
| Mgi Id | MGI:4943795 | Doi | 10.1016/j.bbamcr.2010.07.005 |
| Citation | Jo JO, et al. (2010) Thymosin beta4 induces the expression of vascular endothelial growth factor (VEGF) in a hypoxia-inducible factor (HIF)-1alpha-dependent manner. Biochim Biophys Acta 1803(11):1244-51 |
| abstractText | Thymosin beta4 has multi-functional roles in cell physiology, but little is known about its mechanism(s) of action. We previously reported that thymosin beta4 stimulated angiogenesis through the induction of vascular endothelial growth factor (VEGF). To identify the mechanism of VEGF induction by thymosin beta4, we have used a luciferase assay system with VEGF in the 5' promoter region. We also analyzed the effect of thymosin beta4 on VEGF mRNA stability and on the expression and stability of hypoxia-inducible factor (HIF)-1alpha. We found that thymosin beta4 induces VEGF expression by an increase in the stability of HIF-1alpha protein. Analysis of the expression patterns of thymosin beta4 and HIF-1alpha in colon cancer tissue microarray showed that thymosin beta4 and HIF-1alpha co-localized in these biopsies. These data show that thymosin beta4 induces the expression of VEGF indirectly by increasing the protein stability of HIF-1alpha. |
