| First Author | Aguilar-Morante D | Year | 2011 |
| Journal | Neuroscience | Volume | 176 |
| Pages | 110-9 | PubMed ID | 21185356 |
| Mgi Jnum | J:170577 | Mgi Id | MGI:4946891 |
| Doi | 10.1016/j.neuroscience.2010.12.025 | Citation | Aguilar-Morante D, et al. (2011) Decreased CCAAT/enhancer binding protein beta expression inhibits the growth of glioblastoma cells. Neuroscience 176:110-9 |
| abstractText | C/EBPbeta is a leucine-zipper transcription factor implicated in the control of metabolism, development, cell differentiation, and proliferation. However, it remains unclear its role in tumor development. Here, we show that down-regulation of C/EBPbeta by RNA interference inhibits proliferation in the GL261 murine glioblastoma cell line, induces an arrest of the cell cycle at the G0/G1 boundary, and diminishes their transformation capacity and migration. In addition, we show that C/EBPbeta regulates the expression of several DNA damage response- and invasion-related genes. Lastly, C/EBPbeta depletion significantly retards tumor onset and prolongs survival in a murine orthotopic brain tumor model. Immunohistochemical analysis revealed a significant diminution of proliferating cell nuclear antigen (PCNA) labeling in tumors derived from C/EBPbeta-depleted GL261 cells compared with that in controls. These results show, for the first time, the dependence of glioma cells on C/EBPbeta and suggest a potential role of this transcription factor in glioma development. |
