| First Author | Mu X | Year | 2011 |
| Journal | PLoS One | Volume | 6 |
| Issue | 2 | Pages | e16699 |
| PubMed ID | 21304901 | Mgi Jnum | J:170912 |
| Mgi Id | MGI:4947888 | Doi | 10.1371/journal.pone.0016699 |
| Citation | Mu X, et al. (2011) Study of muscle cell dedifferentiation after skeletal muscle injury of mice with a Cre-Lox system. PLoS One 6(2):e16699 |
| abstractText | BACKGROUND: Dedifferentiation of muscle cells in the tissue of mammals has yet to be observed. One of the challenges facing the study of skeletal muscle cell dedifferentiation is the availability of a reliable model that can confidentially distinguish differentiated cell populations of myotubes and non-fused mononuclear cells, including stem cells that can coexist within the population of cells being studied. METHODOLOGY/PRINCIPAL FINDINGS: In the current study, we created a Cre/Lox-beta-galactosidase system, which can specifically tag differentiated multinuclear myotubes and myotube-generated mononuclear cells based on the activation of the marker gene, beta-galactosidase. By using this system in an adult mouse model, we found that beta-galactosidase positive mononuclear cells were generated from beta-galactosidase positive multinuclear myofibers upon muscle injury. We also demonstrated that these mononuclear cells can develop into a variety of different muscle cell lineages, i.e., myoblasts, satellite cells, and muscle derived stem cells. CONCLUSIONS/SIGNIFICANCE: These novel findings demonstrated, for the first time, that cellular dedifferentiation of skeletal muscle cells actually occurs in mammalian skeletal muscle following traumatic injury in vivo. |
