| First Author | Kim KY | Year | 2011 |
| Journal | Biochem Biophys Res Commun | Volume | 406 |
| Issue | 4 | Pages | 584-9 |
| PubMed ID | 21352814 | Mgi Jnum | J:171955 |
| Mgi Id | MGI:5002437 | Doi | 10.1016/j.bbrc.2011.02.095 |
| Citation | Kim KY, et al. (2011) Inhibitory effect of leptin on rosiglitazone-induced differentiation of primary adipocytes prepared from TallyHO/Jng mice. Biochem Biophys Res Commun 406(4):584-9 |
| abstractText | The effects of leptin on rosiglitazone-induced adipocyte differentiation were investigated in the primary adipocytes prepared from subcutaneous fat of TallyHO/Jng (TallyHO) mouse, a recently developed model animal for type 2 diabetes mellitus (T2DM). The treatment of leptin inhibited the rosiglitazone-induced adipocyte differentiation with a decreased expression of peroxisome proliferator-activated receptor gamma (PPARgamma) a key adipogenic transcription factor, both in mRNA and protein levels. Leptin (10 nM) was sufficient to inhibit the adipocyte differentiation, which seemed to come from increased expression of leptin receptor genes in the fat of TallyHO mice. The inhibition of adipogenesis by leptin was restored by the treatment of inhibitors for extracellular-signal-regulated kinase (ERK) (PD98059) and signal transducer and activator of transcription-1 (STAT1) (fludarabine). Furthermore, in vivo intraperitoneal administration of PD98059 and fludarabine increased the PPARgamma expression in the subcutaneous fat of TallyHO mice. These data suggest that leptin could inhibit the PPARgamma expression and adipocyte differentiation in its physiological concentration in TallyHO mice. |
