| First Author | Kim DY | Year | 2010 |
| Journal | Nucleic Acids Res | Volume | 38 |
| Issue | 20 | Pages | 7068-78 |
| PubMed ID | 20576698 | Mgi Jnum | J:173417 |
| Mgi Id | MGI:5014012 | Doi | 10.1093/nar/gkq569 |
| Citation | Kim DY, et al. (2010) hnRNP Q and PTB modulate the circadian oscillation of mouse Rev-erb alpha via IRES-mediated translation. Nucleic Acids Res 38(20):7068-78 |
| abstractText | The physiological and behavioral circadian rhythms of most creatures are controlled by a harmony of functional relationships between clock genes. In mammals, several core clock genes show rhythmic profiles of their mRNA and protein expression. Among them, Rev-erb alpha functions as a transcriptional repressor, affecting expression patterns of other clock genes. For the continuous and robust oscillation of the molecular clock system, the levels of Rev-erb alpha protein are expected to be tightly regulated with the correct timing. Here, we demonstrate that Rev-erb alpha has an internal ribosomal entry site (IRES) in its 5' untranslated region. Furthermore, we demonstrate that heterogeneous nuclear ribonucleoprotein Q and polypyrimidine tract-binding protein (PTB) modulate the IRES-mediated translation of Rev-erb alpha. We suggest that the rhythmic binding affinity of hnRNP Q to the Rev-erb alpha IRES and the change in PTB cytosolic levels lead to maintenance of the oscillation profile of the Rev-erb alpha protein. |
